T4 Polynucleotide Kinase

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the γ  position of ATP to the 5'-hydroxyl terminus of polynucleotides (double-stranded and single-stranded DNA and RNA) and nucleoside 3'-monophosphate. T4 polynucleotide kinase also catalyzes the removal of 3'-phosphoryl groups from 3'-phosphate-based polynucleotides, deoxynucleoside 3'-monophosphate, and deoxynucleoside 3' -diphosphate. When ADP is present, T4 PNK has 5'-end phosphatase activity, catalyzing the exchange of 5'-P-oligomeric/polymeric nucleotides and ATP terminal 5'-phosphate groups. It is suitable for constructing DNA libraries and producing probes with terminal labeling.

10U/µL

Application
1. End labeling DNA or RNA for probes and DNA sequencing;
2. 5' phosphorylation of DNA/RNA for subsequent ligation;
3. Phosphorylate the 5' end of the 3' phosphorylated mononucleotide to prepare the pNp substrate for addition to the 3' end of DNA or RNA; 
4. Label the 5' end of oligonucleotides with a phosphate group at the 3' end.

Features
Phosphorylation of the 5' end of DNA or RNA for ligation reactions
Terminal labeling of DNA or RNA for use as probes and DNA sequencing