T4 DNA Polymerase 5U/µL

In the presence of templates and primers, T4 DNA Polymerase catalyzes DNA synthesis in the 5→3 direction. This enzyme also has the activity of 3→5 exonuclease which is stronger than E. coli DNA polymerase I. Unlike DNA polymerase I, T4 DNA Polymerase does not have 5→3 exonuclease activity.

Application
1. Removal of 3'- overhangs to form blunt ends;
2. Fill-in of 5'- overhangs to form blunt ends;
3. Labeling DNA probe synthesis through displacement reaction;
4. Synthesis of the second strand during site-directed mutagenesis;
5. PCR product cloning without relying on ligation reactions.

Features
5' →3' polymerase activity
3' →5' exonuclease activity
No 5' →3' exonuclease activity