GDSBIO
T4 DNA Ligase (Fast)
T4 DNA Ligase (Fast)
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T4 DNA Ligase (Fast) is produced by E. coli carrying T4 bacteriophage gene 30. This enzyme catalyzes the formation of phosphodiester bonds between the 5'-phosphate group and the 3'-hydroxyl group of double-helix DNA or RNA. This enzyme can repair single-strand breaks in double-stranded DNA, RNA, or DNA/RNA complexes, and can connect DNA fragments with sticky or blunt ends, but it is inactive for single-stranded nucleic acids. It is mainly used for cloning of restriction enzyme digestion products, gene site-directed mutagenesis, PCR product cloning, linear DNA self-looping, and repair of double-stranded DNA breaks. T4 DNA Ligase (Fast) requires ATP as a cofactor and completes the sticky end ligation reaction at room temperature in only 10 minutes.
5U/µL
Application
1.TA clone
2.NGS adapter ligation
Features
High ligation activity
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