SYBR Green Blue qPCR Mix (Universal ROX+)

The SYBR Green Blue qPCR Mix (Universal ROX+) is a dye-based qPCR Master Mix, mixed with a blue sample indicator and a universal ROX. There is no need to adjust the concentration of ROX for different instruments, making it easy to operate. The innovative hot start mechanism can reduce the interference of primer dimer and other secondary products on the reaction, significantly improve the specificity and amplification efficiency of quantitative PCR, and obtain a wider range of quantitative amplification. Using a new enhancer, the fluctuation of PCR efficiency for various target fragments can be controlled within a minimum range, and repeated freeze-thaw has little effect on the amplification performance. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.

The reaction system of this product can be prepared at room temperature without an ice box. The prepared PCR reaction system can be placed at room temperature for 24 hours and the amplification efficiency remains unchanged.

Application
Gene expression analysis
Low-copy gene detection
Microarray validation
Gene knockdown validation

Features
Contains sample adding indicator to reduce sample adding errors
Compatible with many Real-time systems
Hot-start technology brings high specificity and reproducible amplification
Contains universal ROX reference dye

Using SYBR Green Blue qPCR Mix and five other similar reagents, the same complex DNA fragment from the same template was amplified under identical conditions. The results showed that the melting curve of the SYBR Green Blue qPCR Mix displayed a standard single peak, indicating a more specific product.