1 1

GDSBIO

S1 Nuclease

S1 Nuclease

$73.00 USD
$73.00 USD
Sale Sold out
Shipping calculated at checkout.
Spec.

Concentration: 100 U/µL

Product Description
S1 nuclease can degrade single-stranded nucleic acids, releasing 5'-phosphorylated mononucleotides or oligonucleotides. Its activity on DNA is five times that on RNA. S1 nuclease can also cleave double-stranded DNA (dsDNA) at single-stranded regions caused by nicks, gaps, mismatches, or loops. S1 nuclease exhibits 3'-phosphomonoesterase activity.
This enzyme is a glycoprotein with a carbohydrate content of 18%.

Source
Aspergillus oryzae strain.

Unit Definition

A unit is defined as the amount of enzyme required to produce 1 µg of acid-soluble deoxyribonucleotides in 1 minute at 37°C.

Enzyme activity is determined in the following mixture: 30 mM sodium acetate (pH 4.5), 50 mM NaCl, 0.1 mM ZnCl2, 5% (v/v) glycerol, and 800 µg/mL heat-denatured calf thymus DNA.

Scope of Application
- Removal of single-stranded overhangs from DNA fragments
- Localization of S1 transcripts
- Cleavage of hairpin loops
- In conjunction with Exonuclease III, creating unidirectional deletions within DNA fragments

View full details

Contact Us / Request a Trial

Pre-Sale Reminders

1. Inquiry via Email
For detailed information, contact our sales team or email order@gdsbio.com (or fill out the form above).

2. Inquiry via Customer Service
Use the chat window in the lower‑right corner (AI assistant) to leave your question and contact details. We’ll respond as soon as possible.

3. Shipping & Logistics
All products are shipped from Guangzhou, with English packaging by default.

  • Most items are in stock; some may take a few days to prepare. We’ll ship as soon as ready.
  • Products typically require low‑temperature shipping (foam box + ice packs), which incurs higher freight costs.
  • Primary carrier: FedEx, DHL.