S1 Nuclease

Concentration: 100 U/µL

Product Description
S1 nuclease can degrade single-stranded nucleic acids, releasing 5'-phosphorylated mononucleotides or oligonucleotides. Its activity on DNA is five times that on RNA. S1 nuclease can also cleave double-stranded DNA (dsDNA) at single-stranded regions caused by nicks, gaps, mismatches, or loops. S1 nuclease exhibits 3'-phosphomonoesterase activity.
This enzyme is a glycoprotein with a carbohydrate content of 18%.

Source
Aspergillus oryzae strain.

Unit Definition

A unit is defined as the amount of enzyme required to produce 1 µg of acid-soluble deoxyribonucleotides in 1 minute at 37°C.

Enzyme activity is determined in the following mixture: 30 mM sodium acetate (pH 4.5), 50 mM NaCl, 0.1 mM ZnCl₂, 5% (v/v) glycerol, and 800 µg/mL heat-denatured calf thymus DNA.

Scope of Application
- Removal of single-stranded overhangs from DNA fragments
- Localization of S1 transcripts
- Cleavage of hairpin loops
- In conjunction with Exonuclease III, creating unidirectional deletions within DNA fragments