RNase H

Concentration: 5 U/µL

Product Description
Ribonuclease H (RNase H) can specifically degrade the RNA strand within RNA-DNA hybrids. This enzyme does not hydrolyze the phosphodiester bonds in single-stranded or double-stranded DNA and RNA.

Source
E. coli strain MRE-600.

Unit Definition

A unit is defined as the amount of enzyme required to catalyze the formation of 1 nmol of acid-soluble products within 20 minutes at 37°C.

Enzyme activity is determined in the following mixture: 20 mM Tris-HCl (pH 7.8), 40 mM KCl, 8 mM MgCl₂,1 mM DTT, 24 µM [³H]-poly(A)·poly(dT), 0.03 mg/mL BSA, and 4% (v/v) glycerol.

Scope of Application
- Removal of mRNA before the synthesis of the second-strand cDNA
- RT-PCR and qRT-PCR: Removal of RNA after the first-strand cDNA synthesis
- Removal of poly(A) sequence from mRNA after hybridization with Oligo(dT)
- Site-specific cleavage of RNA
- Study of products from in vitro polyadenylation reactions