GDSBIO
Power Reverse Transcriptase
Power Reverse Transcriptase
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Power Reverse Transcriptase is a super thermostable reverse transcriptase derived from the Moloney murine leukemia virus (M-MLV) through genetic mutation screening. It synthesizes the first-strand cDNA at 42°C-55°C and withstands 65°C, making it ideal for high-temperature reverse transcription. The enzyme lacks RNase H activity and has high tolerance to common reverse transcriptase inhibitors. It is capable of reverse transcribing RNA with complex secondary structures, synthesizing long cDNA, and constructing full-length cDNA libraries with high proportion.
Concentration: 200U/μl
Application
1st strand cDNA synthesis,especially for one-step RT-PCR/qPCR
Features
The optimum activity temperature is 42-55℃
Tolerant to high temperatures up to 65℃
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