One-step Probe RT-qPCR Kit V3

One-step Probe RT-qPCR Kit V3 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55¡ãC, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 5 ¡Á One-step U+ Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity, low template concentration and multiple detection systems with fluorescent labeled probes such as TaqMan. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.

Application
Probe gene expression analysis
Probe Low-copy gene detection
Probe microarray validation
Probe gene knockdown validation

Features
Fast start-up, 30s hot start
Reverse transcription at 55 ¡ãC to accommodate complex RNA templates
Contains heat-labile UDG to reduce false-positive contamination
High sensitivity for detection of low-abundance genes
High stability, unchanged performance at 4¡ãC and 37¡ãC for one week
The reaction system can be formulated up to 30 minutes in advance without changing performance
Saves time by supporting fast program