Multiplex Probe qPCR Mix Plus U (Low ROX+)

Multiplex Probe qPCR Mix Plus U (Low ROX+) is a 2X concentrated premix for real-time quantitative PCR with probe method. In use, just add the DNA template, primer and probe to react. This product contains antibody technology modified Hotstart Taq DNA polymerase. Combined with GDSBio's special real-time PCR Buffer, it can not only effectively inhibit primers dimer and other non-specific amplification, but also improve the amplification efficiency, allowing multi-probe qPCR reaction. This reagent introduced dUTP/UDG anti-contamination system, which can remove PCR products containing dUTP before PCR reaction, effectively avoid the influence of cross contamination of amplification products on quantification. This product is convenient for use with TaqMan, primers to achieve a fully premixed Master Mix molecular diagnostic kit, and is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc. The product has been pre-mixed with low-concentration ROX reference dye, which is suitable for ABI, Roche and other fluorescent quantitative PCR instruments requiring low-concentration ROX for calibration. It can be used with TaqMan and other fluorescent probes.

The reaction system of this product can be prepared at room temperature without an ice box. The prepared PCR reaction system can be placed at room temperature for 24 hours and the amplification efficiency remains unchanged.

Application
Probe gene expression analysis
Multiple Low-copy gene detection
Probe microarray validation
Probe gene knockdown validation

Features
This kit is suitable for Multiplex fluorescence quantification by probe method
This kit is compatible with many real-time systems require low-concentrtion ROX to calibrate
Hot-start technology brings high specificity and reproducible amplification
dUTP/UDG system, effectively prevent PCR product contamination