Multiplex Probe qPCR Mix Plus U

Multiplex Probe qPCR Mix Plus U is a 2X concentrated premix for real-time quantitative PCR with probe method. In use, just add the DNA template, primer and probe to react. This product contains antibody technology modified Hotstart Taq DNA polymerase. Combined with GDSBio's special real-time PCR Buffer, it can not only effectively inhibit primers dimer and other non-specific amplification, but also improve the amplification efficiency, allowing multi-probe qPCR reaction. This reagent introduced dUTP/UDG anti-contamination system, which can remove PCR products containing dUTP before PCR reaction, effectively avoid the influence of cross contamination of amplification products on quantification. This product is convenient for use with TaqMan, primers to achieve a fully premixed Master Mix molecular diagnostic kit, and is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc. This product can be used with TaqMan and other fluorescent probes, and is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-rad, etc.

The reaction system of this product can be prepared at room temperature without an ice box. The prepared PCR reaction system can be placed at room temperature for 24 hours and the amplification efficiency remains unchanged.

Application
Probe gene expression analysis
Multiple Low-copy gene detection
Probe microarray validation
Probe gene knockdown validation

Features
This kit is suitable for Multiplex fluorescence quantification by probe method
This kit is compatible with many real-time systems
Hot-start technology brings high specificity and reproducible amplification
dUTP/UDG system, effectively prevent PCR product contamination

OEM available

VALIDATION DATA
Using human genomic DNA (0.1ng/µl) as a template, gene detection is performed through Multiplex Probe qPCR Mix Plus U. Figures 1 and 2 are the amplification curves detected after the reaction system is prepared (0 hr) and after being placed at room temperature (25°C) for 48 hours (48 hr), respectively; at the same time, a comparative test is conducted with the manufacturer T's similar product, with Figures 3 and 4 being the amplification curves of manufacturer T's assembly completed at 0 hr and 48 hr, respectively. Table 2 shows the Ct values of Multiplex Probe qPCR Mix Plus U and manufacturer T at 0 hr and 48 hr. It can be seen that after the pre-assembled system is placed at room temperature for 48 hours, Multiplex Probe qPCR Mix Plus U still has excellent amplification performance.