M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA strand. This enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. The enzyme is used to synthesize first-strand cDNA up to 5 kb.
Concentration: 200U/¦Ìl

Application
1st strand cDNA synthesis,especially for two-step RT-PCR/qPCR

Features
The optimum activity temperature is 37-42¡ãC