Klenow Fragment（3´→5´ exo-）

Klenow Fragment （3´→5´ exo-）  is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5´→ 3´ exonuclease activity and has mutations which abolish the 3´→5´ exonuclease activity.

Application
1.Generates probes using random primers
2.Random priming labeling
3.dA-tailing at 3' end
4.Second strand cDNA synthesis

Features
Moderate strand displacement activity