KASP PCR Mix

KASP PCR Mix is a 2X PCR Mix solution developed for KASP, containing double-antibody modified hotstart Taq DNA polymerase, dNTPs, buffer and other essential components for PCR amplification (except templates, primers and probes). When used, only templates, primers and probes need to be added into the amplification system, greatly simplifying the operation process, shortening the operation time, and reducing pollution (less sampling times). The reaction system of KASP PCR Mix is specially optimized to reduce the formation of amplification products by mismatched primers and significantly improve the specificity of PCR amplification.

KASP (Kompetitive Allele-Specific PCR) is a technique for genotyping based on allele-specific PCR. This technology utilises a unique form of competitive allele-specific polymerase chain reaction (PCR) that enables bi-allelic scoring of single nucleotide polymorphisms (SNPs) and insertions/deletions (Indels) at specific loci. KASP has the advantages of high throughput, low cost, and strong operability, and has been widely applied in fields such as genetic and improvement research of crop traits.

Application
SNPs detection
InDels detection

Features
Convenient: pre-mixed, saving time and effort, and reducing the possibility of contamination
High specificity: hotstart Taq DNA Polymerase
High stability: the performance is not easy to change
High sensitivity: fast, accurate, and high-throughput genotyping