General RNA Extraction Kit

General RNA Extraction Kit provides a simple method of isolating total RNA from a wide range of sample types and amounts. In general, samples are lysed and then homogenized in the presence of guanidinium isothiocyanate, a chaotropic salt which is capable of protecting the RNA from endogenous RNases. After homogenization, ethanol is added to the sample. The sample is then processed through a spin column containing a clear silica-based membrane where the RNA binds. Any impurities are effectively removed by subsequent washing. The purified total RNA is then eluted in RNase-Free Water and is suitable for use in a variety of downstream applications.

Application
Purified RNA is free of impurities and enzyme inhibitors, and have an A260/280=1.8-2.0, is suitable for applications such as:
Reverse transcription PCR (RT-PCR)
Real-time quantitative PCR (qPCR)
Northern blotting
Nuclease protection assays
RNA amplification for microarray analysis
cDNA library preparation after poly(A)+ selection

Features
Stable yield
Reliable performance of high-quality purified total RNA in downstream applications