GDS dsDNA Fragmentase

GDS dsDNA Fragmentase, a double-stranded DNA fragmentase, produces dsDNA breaks in a time-dependent manner, producing DNA fragments of 50-1,000 bp depending on reaction time. The GDS dsDNA Fragmentase consists of two enzymes, one that randomly generates nicks on dsDNA and the other recognizes the nicked sites and cuts the opposite DNA strand aross from the nick, thus producing dsDNA breaks. The resulting DNA fragment contains short overhangs, 5' -phosphate, and 3' -hydroxyl groups. The random nicking activity of GDS dsDNA Fragmentase has been demonstrated by the preparation of next-generation sequencing libraries. The comparison of the sequencing results between libraries prepared by genomic DNA sheared by GDS dsDNA Fragmentase with mechanically shearing showed that GDS dsDNA Fragmentase does not introduce any detectable bias in the preparation of sequencing libraries, and there is no difference in sequence coverage between the two methods.

Application
Fragmentation of dsDNA.

Features
Random fragmentation can be achieved by adjusting the incubation time