Fast DNA Library Prep Kit V2

Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, and reagents are highly premixed, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.

Application
NGS library preparation for Illumina platform

Features
1 Wide sample compatibility
2 High efficiency in library construction
3 Compatible with PCR-free workflow
4 End Repair & A-Tailing Enzyme Mix and Buffer are provided as one tube