DSPath 4X One-Step Multiplex Master Mix (High ROX+)

DSPath 4X One-Step Multiplex Master Mix (High ROX+) is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets in quantitative instruments that require high concentrations of ROX, and is suitable for both RNA and DNA targets. This master mix is formulated with optimized buffer components to accommodate multiplex amplification of up to four RNA or DNA target sequences in a single reaction. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions.

Application
Probe gene expression analysis
Probe Low-copy gene detection
Probe microarray validation
Probe gene knockdown validation

Features
Detect multiple targets in one reaction
High sensitivity to detect low-copy targets
Tolerance of inhibitors commonly found in clinical samples
Eliminates the risk of cross contamination associated with two-step RT-qPCR protocols
Premixed with high concentration of ROX reference dye