One-step Probe RT-qPCR Kit

One-step Probe RT-qPCR Kit is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55℃, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 2X One-step U+ Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan?. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.

Application
RNA virus & pathogen detection – e.g., SARS-CoV-2, HIV, influenza, dengue, HCV
Gene expression quantification – from low-abundance or precious RNA samples
Multiplex target detection – simultaneous detection of 4–6 targets in one tube
Direct amplification (no RNA extraction) – using crude lysates from swabs, blood, tissues
Low-abundance & high-GC content RNA templates – high sensitivity down to a few copies or 0.1 pg total RNA

Features
One-step RT-qPCR
This kit is suitable for fluorescence quantification by probe method
This kit is compatible with many real-time systems
Hot-start technology brings high specificity and reproducible amplification
dUTP/UDG system, effectively prevent PCR product contamination