BR Grade. For Research Use Only Specific Activity: ≥3000 U/mg protein (≥60 Kunitz units/mg protein). Form: lyophilized powder
The RNase A is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate.
Application --Plasmid and genomic DNA preparation --Removal of RNA from recombinant protein preparations. --Ribonuclease protection assays --Mapping single-base mutations in DNA or RNA
