GDSBIO
Fragmento de Klenow (exo-3´→5´)
Fragmento de Klenow (exo-3´→5´)
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El fragmento Klenow (3´→5´ exo-) es un truncamiento N-terminal de la ADN polimerasa I que conserva la actividad de polimerasa, pero ha perdido la actividad de exonucleasa 5´→ 3´ y tiene mutaciones que eliminan la actividad de exonucleasa 3´→5´.
Solicitud
1. Genera sondas utilizando cebadores aleatorios
2. Etiquetado de cebado aleatorio
3.dA-cola en el extremo 3'
4.Síntesis de ADNc de segunda cadena
Características
Actividad moderada de desplazamiento de hebras
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