GDSLock Hotstart Taq Polymerase

GDSLock Hotstart Taq Polymerase is a ligand-modified hot-start DNA polymerase utilizing covalent chemical blocking technology. Through reversible covalent binding of specific small-molecule ligands to the enzyme's active center, the polymerase activity is blocked by ≥99% at ambient temperature, completely eliminating unintended extension caused by non-specific primer annealing or primer-dimer formation during reaction setup and thermal ramping.

Unlike antibody-based hot-start enzymes, this product is animal-origin-free, completely avoiding the potential introduction of animal-derived protein contaminants associated with antibody-based methods. It is the ideal choice for IVD kit manufacturing and forensic DNA analysis.

Upon heating to 95°C, the ligand molecules are slowly released and dissociated within 1-5 minutes, with enzymatic activity gradually restored to the level of native Taq polymerase. This "time-released thermal activation" mechanism ensures stringent low-temperature blocking while preventing potential substrate competition caused by instantaneous release, thereby further enhancing amplification uniformity.

GDSLock Hotstart Taq Polymerase possesses 5’-3’ polymerase activity, lacks 3’-5’ exonuclease activity, and produces amplicons with 3’-dA overhangs.

Product Features
Reversible ligand modification for precise hot-start activation
Ambient-temperature blocking efficiency ≥99%, eliminating non-specific amplification
Rapid hot-start activation, fully activated within 1–5 minutes
Sustained heat-activated release, providing excellent amplification uniformity
High sensitivity, enabling stable detection of low-copy templates
High specificity, with clear advantages in complex template amplification
Animal-origin free, meeting IVD and forensic-grade quality standards
Excellent batch-to-batch consistency and stable quality

Applications
IVD kit production
Forensic DNA analysis
Low-copy-number / trace DNA template detection
Multiplex PCR amplification
Routine and rapid endpoint PCR
High-GC-content and complex template amplification
Real-time quantitative PCR (SYBR Green / dye-based methods)
Automated high-throughput PCR setups