Direct One-step Probe RT-qPCR Kit

Direct One-step Probe RT-qPCR Kit is a nucleic acid extraction-free, one-step probe-based RT-qPCR master mix. The Direct One-step Enzyme Mix contains optimized reverse transcriptase, antibody-modified hot-start Taq, and temperature-sensitive UDG enzyme, which eliminates DNA contamination at room temperature and is inactivated during the reverse transcription step. This product exhibits extremely high tolerance to inhibitors and can be used directly for target gene detection in various blood samples, swabs, and saliva. It is compatible with multiplex fluorescent PCR systems, supports fast protocols, and is suitable for pathogen detection, gene expression analysis, and other research and IVD development applications.

Applications
IVD product development for direct amplification of RNA virus samples and tumor samples.
One-step RT-qPCR experiments for multiple RNA targets.

Features

No nucleic acid extraction required: Directly compatible with crude samples (e.g., whole blood, serum, saliva).

High inhibitor tolerance: Robust performance in complex sample matrices.

Supports fast cycling protocols: Ideal for rapid pathogen detection.

Room-temperature UDG treatment: Prevents DNA contamination effectively.

Suitable for IVD development and research: Especially for RNA virus detection and direct amplification from tumor samples.