{"product_id":"gdslock-hotstart-taq-polymerase","title":"GDSLock Hotstart Taq Polymerase","description":"\u003cp\u003eGDSLock Hotstart Taq Polymerase is a ligand-modified hot-start DNA polymerase utilizing covalent chemical blocking technology. Through reversible covalent binding of specific small-molecule ligands to the enzyme's active center, the polymerase activity is blocked by ≥99% at ambient temperature, completely eliminating unintended extension caused by non-specific primer annealing or primer-dimer formation during reaction setup and thermal ramping.\u003c\/p\u003e\n\u003cp\u003eUnlike antibody-based hot-start enzymes, this product is animal-origin-free, completely avoiding the potential introduction of animal-derived protein contaminants associated with antibody-based methods. It is the ideal choice for IVD kit manufacturing and forensic DNA analysis.\u003c\/p\u003e\n\u003cp\u003eUpon heating to 95°C, the ligand molecules are slowly released and dissociated within 1-5 minutes, with enzymatic activity gradually restored to the level of native Taq polymerase. This \"time-released thermal activation\" mechanism ensures stringent low-temperature blocking while preventing potential substrate competition caused by instantaneous release, thereby further enhancing amplification uniformity.\u003c\/p\u003e\n\u003cp\u003eGDSLock Hotstart Taq Polymerase possesses 5’-3’ polymerase activity, lacks 3’-5’ exonuclease activity, and produces amplicons with 3’-dA overhangs.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Features\u003c\/strong\u003e\u003cbr\u003eReversible ligand modification for precise hot-start activation\u003cbr\u003eAmbient-temperature blocking efficiency ≥99%, eliminating non-specific amplification\u003cbr\u003eRapid hot-start activation, fully activated within 1–5 minutes\u003cbr\u003eSustained heat-activated release, providing excellent amplification uniformity\u003cbr\u003eHigh sensitivity, enabling stable detection of low-copy templates\u003cbr\u003eHigh specificity, with clear advantages in complex template amplification\u003cbr\u003eAnimal-origin free, meeting IVD and forensic-grade quality standards\u003cbr\u003eExcellent batch-to-batch consistency and stable quality\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cbr\u003eIVD kit production\u003cbr\u003eForensic DNA analysis\u003cbr\u003eLow-copy-number \/ trace DNA template detection\u003cbr\u003eMultiplex PCR amplification\u003cbr\u003eRoutine and rapid endpoint PCR\u003cbr\u003eHigh-GC-content and complex template amplification\u003cbr\u003eReal-time quantitative PCR (SYBR Green \/ dye-based methods)\u003cbr\u003eAutomated high-throughput PCR setups\u003c\/p\u003e","brand":"GDSBio","offers":[{"title":"P1291\/250U","offer_id":44215572463750,"sku":"P1291","price":43.0,"currency_code":"USD","in_stock":true},{"title":"P1292\/1000U","offer_id":44215572496518,"sku":"P1292","price":154.0,"currency_code":"USD","in_stock":true},{"title":"P1293\/5000U","offer_id":44215572529286,"sku":"P1293","price":692.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_4649c787-5da2-45b4-8e86-499ab660134b.jpg?v=1745743852","url":"https:\/\/gdsbio.com\/es\/products\/gdslock-hotstart-taq-polymerase","provider":"GDSBio","version":"1.0","type":"link"}