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Fast Restriction Endonuclease SacI For Molecular Cloning
Product Details:
| Place of Origin: | China |
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | E1037 |
Payment & Shipping Terms:
| Minimum Order Quantity: | 100 rxns |
|---|---|
| Price: | USD 96.8-107.5/100 rxns |
| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8 work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
|
Detail Information |
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| Product Name: | SacI | Cat. No./Spec.: | E1037-A/100 Rxns |
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| Appearance: | Colourless | Application: | Molecular Cloning |
| Classification: | General Reagents | Grade: | Molecular Biology |
| Logo Printing: | With Logo Printing | Transport Package: | Packing |
| Production Capacity: | 100 Bag/Bags Per Day | Storage Conditions: | Store At -20°C |
| High Light: | fast restriction endonuclease SacI,Endonuclease SacI For Molecular Cloning |
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Product Description
SacⅠ
Cat. No./Spec.: E1037-A/100 rxns
Recognition site:
5' G A G C T ↓ C 3'
3' C ↑ T C G A G 5'
Product Description
The SacⅠ restriction endonuclease recognizes the GAGCT^C site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer. Isoschizomers: Eco53kⅠ, EcoICRⅠ, Psp124BⅠ, SstⅠ.
GDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.
The universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.
The Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.
Cat. No./Spec.: E1037-A/100 rxns
Recognition site:
5' G A G C T ↓ C 3'
3' C ↑ T C G A G 5'
Product Description
The SacⅠ restriction endonuclease recognizes the GAGCT^C site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer. Isoschizomers: Eco53kⅠ, EcoICRⅠ, Psp124BⅠ, SstⅠ.
GDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.
The universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.
The Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.
Storage Condition & Shelf Life
Store at -20°C.
Features
- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.
- 100% buffer compatibility with downstream applications.
- Enzymatic digestion can be completed within 5–15 minutes.
- Direct loading onto a gel.
- No star activity.
Scope of Application
- Molecular cloning
- Restriction site mapping
- Genotyping
- Southern blotting
- Restriction fragment length polymorphism (RFLP)
- SNP (Single Nucleotide Polymorphism) analysis
Store at -20°C.
Features
- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.
- 100% buffer compatibility with downstream applications.
- Enzymatic digestion can be completed within 5–15 minutes.
- Direct loading onto a gel.
- No star activity.
Scope of Application
- Molecular cloning
- Restriction site mapping
- Genotyping
- Southern blotting
- Restriction fragment length polymorphism (RFLP)
- SNP (Single Nucleotide Polymorphism) analysis
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