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Professional T4 DNA Polymerase #K011
Product Details:
| Place of Origin: | China |
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | K011 |
Payment & Shipping Terms:
| Minimum Order Quantity: | 100U |
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| Price: | inquiry |
| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8 work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
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Detail Information |
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| Cat. No.: | K011-A | Specification: | 100U |
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| Appearance: | Colourless | Application: | DNA End Repair |
| Classification: | General Reagents | Grade: | Molecular Biology |
| Logo Printing: | With Logo Printing | Transport Package: | Packing |
| Production Capacity: | 100 Bag/Bags Per Day | Storage Conditions: | Store At -20°C |
| High Light: | K011 T4 DNA Polymerase,T4 DNA Polymerase |
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Product Description
Cat. No./Spec.: K011-A/100U; K011-B/500U; K011-C/2000U; K011-D/5000U
Concentration: 5U/µL
Product Description
In the presence of templates and primers, T4 DNA Polymerase catalyzes DNA synthesis in the 5 ́→3 ́ direction. This enzyme also has the activity of 3 ́→5 ́ exonuclease which is stronger than E. coli DNA polymerase I. Unlike DNA polymerase I, T4 DNA Polymerase does not have 5 ́→3 ́ exonuclease activity.
Components
| Component | K011-A (100U) | K011-B (500U) | K011-C (2,000U) | K011-D (5,000U) |
| T4 DNA Polymerase (5U/µL) | 20 µL | 100 µL | 400 µL | 1 mL |
| 10X Blue Buffer | 100 µL | 250 µL | 500 µL | 1.25 mL |
Storage Condition
All reagents should be stored at -20°C. The product is valid for 12 months.
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.
Scope of Application
1. Removal of 3’ overhangs to form blunt ends;
- Fill-in of 5’ overhangs to form blunt ends;
- Labeling DNA probe synthesis through displacement reaction;
- Synthesis of the second strand during site-directed mutagenesis;
- PCR product cloning without relying on ligation reactions.
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